caspase 3 substrate ac devd afc fluorometric assay kit Search Results


caspase 3 9 activity assay kit  (Beyotime)
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Caspase 3 9 Activity Assay Kit, supplied by Beyotime, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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caspase 3/7 activity kit  (AAT Bioquest)
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AAT Bioquest caspase 3/7 activity kit
Caspase 3/7 Activity Kit, supplied by AAT Bioquest, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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caspase 3 fluorometric protease assay kit  (MBL Life science)
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MBL Life science caspase 3 fluorometric protease assay kit
Caspase 3 Fluorometric Protease Assay Kit, supplied by MBL Life science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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caspase3/cpp32 fluorimetric assay kit  (GENTAUR Inc)
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nucview 488 assay kit  (Biotium)
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caspase-3 cellular activity assay kit plus  (Biomol GmbH)
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caspase-3 assay kit (colorimetric), 100 test kit  (Advisains)
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Advisains caspase-3 assay kit (colorimetric), 100 test kit
Caspase 3 Assay Kit (Colorimetric), 100 Test Kit, supplied by Advisains, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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caspase 3/7 assay kit  (eEnzyme Inc)
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eEnzyme Inc caspase 3/7 assay kit
Phenotype of TRAP1 expression in Pten+/− prostate epithelial cells. A, primary prostatic epithelial cells were isolated from TRAP1 Tg mice and analyzed for TRAP1 expression by quantitative PCR at the indicated passages (P) in culture. B, Pten+/− prostate epithelial P8 cells transfected with vector or TRAP1 cDNA were incubated in the presence of staurosporine (1 μm) and analyzed for cell death by direct cell counting. C, Pten−/− prostate epithelial CapP8 cells or P8 cells transfected as in B were incubated with etoposide (64 nm) and analyzed for <t>caspase-3/7</t> activity. D—H, P8 cells transfected as in B were analyzed for cell proliferation by direct cell counting on days 5 and 7 after transfection (D), cell invasion across Matrigel-coated inserts (E), ATP/ADP ratio (F), OCR in the presence or absence of the mitochondrial complex I inhibitor rotenone (Rot, 1 μm) (G), or mitochondrial superoxide production (H). *, p = 0.01–0.04; ***, p < 0.0001. H2O2 was used as a control oxidative stimulus.
Caspase 3/7 Assay Kit, supplied by eEnzyme Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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caspase  (Novus Biologicals)
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Novus Biologicals caspase
Phenotype of TRAP1 expression in Pten+/− prostate epithelial cells. A, primary prostatic epithelial cells were isolated from TRAP1 Tg mice and analyzed for TRAP1 expression by quantitative PCR at the indicated passages (P) in culture. B, Pten+/− prostate epithelial P8 cells transfected with vector or TRAP1 cDNA were incubated in the presence of staurosporine (1 μm) and analyzed for cell death by direct cell counting. C, Pten−/− prostate epithelial CapP8 cells or P8 cells transfected as in B were incubated with etoposide (64 nm) and analyzed for <t>caspase-3/7</t> activity. D—H, P8 cells transfected as in B were analyzed for cell proliferation by direct cell counting on days 5 and 7 after transfection (D), cell invasion across Matrigel-coated inserts (E), ATP/ADP ratio (F), OCR in the presence or absence of the mitochondrial complex I inhibitor rotenone (Rot, 1 μm) (G), or mitochondrial superoxide production (H). *, p = 0.01–0.04; ***, p < 0.0001. H2O2 was used as a control oxidative stimulus.
Caspase, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fitc active caspase-3 apoptosis kit  (Becton Dickinson)
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Becton Dickinson fitc active caspase-3 apoptosis kit
Phenotype of TRAP1 expression in Pten+/− prostate epithelial cells. A, primary prostatic epithelial cells were isolated from TRAP1 Tg mice and analyzed for TRAP1 expression by quantitative PCR at the indicated passages (P) in culture. B, Pten+/− prostate epithelial P8 cells transfected with vector or TRAP1 cDNA were incubated in the presence of staurosporine (1 μm) and analyzed for cell death by direct cell counting. C, Pten−/− prostate epithelial CapP8 cells or P8 cells transfected as in B were incubated with etoposide (64 nm) and analyzed for <t>caspase-3/7</t> activity. D—H, P8 cells transfected as in B were analyzed for cell proliferation by direct cell counting on days 5 and 7 after transfection (D), cell invasion across Matrigel-coated inserts (E), ATP/ADP ratio (F), OCR in the presence or absence of the mitochondrial complex I inhibitor rotenone (Rot, 1 μm) (G), or mitochondrial superoxide production (H). *, p = 0.01–0.04; ***, p < 0.0001. H2O2 was used as a control oxidative stimulus.
Fitc Active Caspase 3 Apoptosis Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse caspase-3 elisa kit  (MyBiosource Biotechnology)
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MyBiosource Biotechnology mouse caspase-3 elisa kit
Phenotype of TRAP1 expression in Pten+/− prostate epithelial cells. A, primary prostatic epithelial cells were isolated from TRAP1 Tg mice and analyzed for TRAP1 expression by quantitative PCR at the indicated passages (P) in culture. B, Pten+/− prostate epithelial P8 cells transfected with vector or TRAP1 cDNA were incubated in the presence of staurosporine (1 μm) and analyzed for cell death by direct cell counting. C, Pten−/− prostate epithelial CapP8 cells or P8 cells transfected as in B were incubated with etoposide (64 nm) and analyzed for <t>caspase-3/7</t> activity. D—H, P8 cells transfected as in B were analyzed for cell proliferation by direct cell counting on days 5 and 7 after transfection (D), cell invasion across Matrigel-coated inserts (E), ATP/ADP ratio (F), OCR in the presence or absence of the mitochondrial complex I inhibitor rotenone (Rot, 1 μm) (G), or mitochondrial superoxide production (H). *, p = 0.01–0.04; ***, p < 0.0001. H2O2 was used as a control oxidative stimulus.
Mouse Caspase 3 Elisa Kit, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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caspase-3 elisa kit  (MyBiosource Biotechnology)
90
MyBiosource Biotechnology caspase-3 elisa kit
Phenotype of TRAP1 expression in Pten+/− prostate epithelial cells. A, primary prostatic epithelial cells were isolated from TRAP1 Tg mice and analyzed for TRAP1 expression by quantitative PCR at the indicated passages (P) in culture. B, Pten+/− prostate epithelial P8 cells transfected with vector or TRAP1 cDNA were incubated in the presence of staurosporine (1 μm) and analyzed for cell death by direct cell counting. C, Pten−/− prostate epithelial CapP8 cells or P8 cells transfected as in B were incubated with etoposide (64 nm) and analyzed for <t>caspase-3/7</t> activity. D—H, P8 cells transfected as in B were analyzed for cell proliferation by direct cell counting on days 5 and 7 after transfection (D), cell invasion across Matrigel-coated inserts (E), ATP/ADP ratio (F), OCR in the presence or absence of the mitochondrial complex I inhibitor rotenone (Rot, 1 μm) (G), or mitochondrial superoxide production (H). *, p = 0.01–0.04; ***, p < 0.0001. H2O2 was used as a control oxidative stimulus.
Caspase 3 Elisa Kit, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Phenotype of TRAP1 expression in Pten+/− prostate epithelial cells. A, primary prostatic epithelial cells were isolated from TRAP1 Tg mice and analyzed for TRAP1 expression by quantitative PCR at the indicated passages (P) in culture. B, Pten+/− prostate epithelial P8 cells transfected with vector or TRAP1 cDNA were incubated in the presence of staurosporine (1 μm) and analyzed for cell death by direct cell counting. C, Pten−/− prostate epithelial CapP8 cells or P8 cells transfected as in B were incubated with etoposide (64 nm) and analyzed for caspase-3/7 activity. D—H, P8 cells transfected as in B were analyzed for cell proliferation by direct cell counting on days 5 and 7 after transfection (D), cell invasion across Matrigel-coated inserts (E), ATP/ADP ratio (F), OCR in the presence or absence of the mitochondrial complex I inhibitor rotenone (Rot, 1 μm) (G), or mitochondrial superoxide production (H). *, p = 0.01–0.04; ***, p < 0.0001. H2O2 was used as a control oxidative stimulus.

Journal: The Journal of Biological Chemistry

Article Title: Transgenic Expression of the Mitochondrial Chaperone TNFR-associated Protein 1 (TRAP1) Accelerates Prostate Cancer Development *

doi: 10.1074/jbc.M116.745950

Figure Lengend Snippet: Phenotype of TRAP1 expression in Pten+/− prostate epithelial cells. A, primary prostatic epithelial cells were isolated from TRAP1 Tg mice and analyzed for TRAP1 expression by quantitative PCR at the indicated passages (P) in culture. B, Pten+/− prostate epithelial P8 cells transfected with vector or TRAP1 cDNA were incubated in the presence of staurosporine (1 μm) and analyzed for cell death by direct cell counting. C, Pten−/− prostate epithelial CapP8 cells or P8 cells transfected as in B were incubated with etoposide (64 nm) and analyzed for caspase-3/7 activity. D—H, P8 cells transfected as in B were analyzed for cell proliferation by direct cell counting on days 5 and 7 after transfection (D), cell invasion across Matrigel-coated inserts (E), ATP/ADP ratio (F), OCR in the presence or absence of the mitochondrial complex I inhibitor rotenone (Rot, 1 μm) (G), or mitochondrial superoxide production (H). *, p = 0.01–0.04; ***, p < 0.0001. H2O2 was used as a control oxidative stimulus.

Article Snippet: In other experiments, P8 or CapP8 cells transfected with vector or TRAP1 cDNA were plated in triplicates (5 × 10 3 ) onto 96-well black plates in 100 μl of complete DMEM without phenol red, exposed to etoposide (64 n m ), and assayed after 5 h for changes in terminal caspase-3/7 activity using a caspase 3/7 assay kit (eEnzyme, catalog no. CA-C150).

Techniques: Expressing, Isolation, Real-time Polymerase Chain Reaction, Transfection, Plasmid Preparation, Incubation, Cell Counting, Activity Assay